lenti cas9 blast (Addgene inc)
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Addgene inc
lenti cas9 blast
Lenti Cas9 Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticas9+blast/lentiCas9-Blast+(Plasmid+%2352962)/pm41932441-250-16-17
Average 96 stars, based on 1651 article reviews
Lenti Cas9 Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticas9+blast/lentiCas9-Blast+(Plasmid+%2352962)/pm41932441-250-16-17
Average 96 stars, based on 1651 article reviews
lenti cas9 blast - by Bioz Stars,
2026-10
96/100 stars
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Transduction:Article Title: Context-dependent determinants of CRISPR-Cas9 editing efficiency revealed through cross-species endogenous editing analysis Article Snippet: NGS library preparation was performed at 8 pM concentration with 20% PhiX, and sequencing was conducted on a MiSeq platform with a 2x151 read configuration. .. U937 cells (a kind gift from Prof Ofer Medelboim) were transduced with Article Title: Large-scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation. Article Snippet: After transfection, cells were subjected to antibiotic selection with Blasticidin S HCl (10 μg mL 1, Catalog #A1113903; Thermo Fisher Scientific) to establish a stable dCas9-expressing cell population. .. Similarly, Cas9-expressing cells were generated via lentiviral transduction using Article Title: The allosteric IDH1 inhibitor ivosidenib overcomes chemoresistance in intrahepatic cholangiocarcinoma models expressing wild-type IDH1 Article Snippet: 632 633 CRISPR/Cas9 library screening 634 To investigate the potential crucial targets that facilitate resistance of iCCA to GEM 635 treatment, we constructed a CRISPR/Cas9 library containing 7173 druggable genes at 636 32 a coverage of four gRNAs per gene as well as 1308 non-targeting control gRNAs as 637 previously described (33-35), which was designed following the instructions of GPP 638 sgRNA Designer of BROAD Institute (portals.broadinstitute.org/gppx/crispick/public). .. 639 HuCCT1, the iCCA cell line with primary resistance to GEM, was transduced with 640 Generated:Article Title: Locus-specific proteomics identifies novel regulators of Epstein-Barr virus lytic reactivation. Article Snippet: .. The pCEP4-Cas9 vector was generated by cloning the Cas9 sequence from Article Title: Mutant ribosomal protein RPS15 drives B cell malignancy through oxidative stress and genomic instability. Article Snippet: .. AR TI CL E IN P RE SS To generate cells compatible with the CRISPR-Cas9 system, lentivirus was generated by cotransfection of Article Title: Large-scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation. Article Snippet: After transfection, cells were subjected to antibiotic selection with Blasticidin S HCl (10 μg mL 1, Catalog #A1113903; Thermo Fisher Scientific) to establish a stable dCas9-expressing cell population. .. Similarly, Cas9-expressing cells were generated via lentiviral transduction using Cloning:Article Title: Locus-specific proteomics identifies novel regulators of Epstein-Barr virus lytic reactivation. Article Snippet: .. The pCEP4-Cas9 vector was generated by cloning the Cas9 sequence from Sequencing:Article Title: Locus-specific proteomics identifies novel regulators of Epstein-Barr virus lytic reactivation. Article Snippet: .. The pCEP4-Cas9 vector was generated by cloning the Cas9 sequence from Expressing:Article Title: Fidelity-Ensuring Consistency of Mitosis is Safeguarded by the 53BP1-USP28-p53 Pathway Article Snippet: .. Stable expression of Cas9 was established using the Plasmid Preparation:Article Title: Fidelity-Ensuring Consistency of Mitosis is Safeguarded by the 53BP1-USP28-p53 Pathway Article Snippet: .. Stable expression of Cas9 was established using the other:Article Title: CDK12/CDK13 inhibition disrupts transcriptional elongation and replication fork progression in glioblastoma Article Snippet: Organoid morphology was monitored with the Incucyte system, and viability was assessed using CellTiter-Glo 3D (Promega); luminescence was read on a ClarioStar (BMG Labtech). CRISPR:Article Title: Mutant ribosomal protein RPS15 drives B cell malignancy through oxidative stress and genomic instability. Article Snippet: .. AR TI CL E IN P RE SS To generate cells compatible with the CRISPR-Cas9 system, lentivirus was generated by cotransfection of Cotransfection:Article Title: Mutant ribosomal protein RPS15 drives B cell malignancy through oxidative stress and genomic instability. Article Snippet: .. AR TI CL E IN P RE SS To generate cells compatible with the CRISPR-Cas9 system, lentivirus was generated by cotransfection of Selection:Article Title: Large-scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation. Article Snippet: After transfection, cells were subjected to antibiotic selection with Blasticidin S HCl (10 μg mL 1, Catalog #A1113903; Thermo Fisher Scientific) to establish a stable dCas9-expressing cell population. .. Similarly, Cas9-expressing cells were generated via lentiviral transduction using Western Blot:Article Title: The allosteric IDH1 inhibitor ivosidenib overcomes chemoresistance in intrahepatic cholangiocarcinoma models expressing wild-type IDH1 Article Snippet: 632 633 CRISPR/Cas9 library screening 634 To investigate the potential crucial targets that facilitate resistance of iCCA to GEM 635 treatment, we constructed a CRISPR/Cas9 library containing 7173 druggable genes at 636 32 a coverage of four gRNAs per gene as well as 1308 non-targeting control gRNAs as 637 previously described (33-35), which was designed following the instructions of GPP 638 sgRNA Designer of BROAD Institute (portals.broadinstitute.org/gppx/crispick/public). .. 639 HuCCT1, the iCCA cell line with primary resistance to GEM, was transduced with 640 |